Non-migrated cells were removed having a cotton swab, and invaded cells were fixed in 4% paraformaldehyde for 15 min at room temperature prior to staining with crystal violet. inhibiting the manifestation of the mesenchymal markers N-cadherin and vimentin. … Continue reading Non-migrated cells were removed having a cotton swab, and invaded cells were fixed in 4% paraformaldehyde for 15 min at room temperature prior to staining with crystal violet